以白花泡桐优树'白优2号’为试验材料,通过组织培养和试管嫁接方法,对白花泡桐优树材料的幼化技术进行了研究。结果表明:外植体初代培养萌发的嫩芽为最适合的接穗;'建始桐3号’为试管嫁接较合适的砧木;采用劈接进行;MS+NAA 0.3 mg·L-1 +蔗糖30 g·L-1为试管嫁接培养基; 继代增殖和生根培养基分别为1/2MS+6-BA 6.0 mg·L -1 + NAA 0.3 mg·L-1+蔗糖20 g·L-1和1/2MS+NAA 0.1 mg·L-1。

The Castanopsis sclerophylla seed length, seed diameter, seed length× seed diameter, seed length/seed diameter and hundred grain weight from nine areas were studied. The results showed that extremely significant difference were found in seed characters of C. sclerophylla from different areas. The most coefficient of variation is hundred grain weight and the least is seed length/seed diameter. Correlation analysis showed that the seed length and seed diameter had the significant positive correlation with hundred grain weight; seed became bigger and heavier as latitude became lower, the annual average temperature and frost free period become longer; the hundred grain weight had significant positive correlation with rainfall, seed length/seed diameter had significant negative correlation with the rainfall, And clustering analysis showed that phenotypic variation was basicaly continuous variation with the geography, which was consistent with the result of relevant analysis.

[目的]为了构建松材线虫(Bursaphelenchus xylophilus)果胶酶Bxpel2基因干扰载体.[方法]通过Trizol 法提取松材线虫总RNA,反转录合成cDNA,设计带T7启动子的果胶酶Bxpel2基因引物,以cDNA为模板扩增出果胶酶Bxpel2基因片段,连接到RNA干扰载体,再以干扰载体为模板,PCR扩增出目的片段后进行测序鉴定,合成果胶酶Bxpel2基因双链RNA(dsRNA),采用RT-PCR检测松材线虫Bxpel2基因干扰后的表达情况.[结果]表明:1)提取的松材线虫总RNA完整性好,无降解;2)成功克隆出松材线虫果胶酶Bxpel2基因片段(790 bp)并将其连接至pMD19-T载体;3)以RNA干扰载体为模板合成dsRNA,浓度分别为1.313 mg·mL-1和1.152 mg·mL-1;4)RT-PCR结果显示,松材线虫经过dsRNA干扰后,Bxpel2基因表达基本受到抑制.[结论]成功构建松材线虫果胶酶Bxpel2基因干扰载体,为进一步研究Bxpel2基因在松材线虫致病过程中的作用和功能奠定了良好的基础.

Xanthoceras sorbifolia provenances were obtained in their main districts, and some cone characters were tested which includes cone diameter(A), cone length(B), pericarp thickness(C), fruitstalk diameter(D), single cone weight(E), pericarp weight(F), seed weight(G), number of seed room(H), seed number(J), rate of seed of cone(K). From the analysis of those characters, it was found that the indexes of characters of single cone weight(E), seed weight(G), pericarp weight(F) had large disparities, and the indexes of characters of cone diameter(A), cone length(B), pericarp thickness(C), fruitstalk diameter(D) had small disparities. There were linearly positive correlations between indexes of characters, the relative coefficient of factors with A is E＞B＞F＞G＞D＞J＞C; the relative coefficient of factors with B is E>G>F>A>J>D>C;the relative coefficient of factors with C is J＞G＞E＞B＞F＞A;the relative coefficient of factors with D is E＞G＞F＞A＞B＞J;the relative coefficient of factors with E is F＞G＞A＞B＞J＞D＞C; the relative coefficient of factors with F is E＞G＞A＞B＞J＞D＞C;the relative coefficient of factors with G is E＞F＞B＞A＞J＞D＞C.